Coding

Part:BBa_K3195003

Designed by: JingYuan Liu   Group: iGEM19_SEU-Nanjing-China   (2019-09-23)
Revision as of 10:08, 5 October 2019 by Bread (Talk | contribs)


His-tagged Cathepsin B

This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.

Usage and Biology


Characterization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 369
    Illegal XhoI site found at 958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//function/degradation
Parameters
originBranchiostoma belcheri tsingtauense
tag6X His