Measurement

Part:BBa_J364000:Experience

Designed by: Traci Haddock-Angelli   Group: Measurement_Lab   (2017-04-19)
Revision as of 23:52, 1 October 2019 by Kristiturton (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J364000

User Reviews

UNIQc023f4d0bafb5c9a-partinfo-00000000-QINU UNIQc023f4d0bafb5c9a-partinfo-00000001-QINU


SCU-China 2018:

Experiment conditions:

1. 37℃ incubator for E.coli DH5α;

2. LB liquid medium.

Analyze & Results:

We use this part BBa_J364000 as our original source of GFP proteins. As our expectation, this part can work normally under our experiment conditions.

For the modification, we added a spacer sequence and NGG site just on the upstream of the promoter for repression of GFP expression. And this modification is very successful because the repression is obvious according to the Figure 1 below.

SCU China-2018 dCas9 repression.png

As the picture shows, the part:BBa_J364000 and our part:BBa_K2611001 can both function well. And for the improvement: this part's expression can be regulated under the control of dCas9 system.

2017 Georgia State iGEM Team

Georgia State Growth Medium Study

Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364000 in multiple growth mediums.

2019 Lethbridge iGEM Team

LB and minimal growth study for BBa_J364000, BBa_J364001 and BBa_J364002.

In order to inform iGEM teams, we have compared these three parts in order to determine their growth and effectiveness in different conditions with the E. coli host. Each was test in LB and M9 minimal media to determine which part has the most resilience. Data was represented in colony forming units by using the microsphere calibration protocol from the 2018 measurement study. This will aid iGEM teams that will work in stress conditions for their projects.

please see our <a href="https://2019.igem.org/wiki/images/c/cd/T--Lethbridge--characterization_data_Lethbridge_2019.pdf">final report</a> to gain information on our media recipes, protocol and results.