Coding

Part:BBa_K2653003

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-10)
Revision as of 02:36, 29 September 2019 by Petersberg (Talk | contribs)

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P2A

The bicistronic P2A peptide is a kind of self-cleaved sequence, which breaks when translated. The protein expressed by the same mRNA is separated before and after to produce two proteins, which can be used to construct the polycis vector and realize the co-translation of two proteins under the control of the same promoter


Usage and Biology

Self-cleavage Bicistronic or multicistronic are widely used in expression of more than one gene at the same time. These molecules shapes only a small size, but has amazingly high cleavage efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




References

[1]Jin H K, Lee S R, Li L H, et al. High Cleavage Efficiency of a 2A Peptide Derived from Porcine, Teschovirus-1 in Human Cell Lines, Zebrafish and Mice[J]. Plos One, 2011, 6(4):e18556.

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