Composite

Part:BBa_K2916049

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-25)
Revision as of 20:46, 25 September 2019 by Konstantinos (Talk | contribs)


Expression of T7 RNA in E.coli

This part is used for expression of T7 RNAP needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 1556
    Illegal NgoMIV site found at 2003
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None