Coding
MK

Part:BBa_K2916011:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-24)
Revision as of 14:18, 24 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


CK protein equipped with a 6x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 821
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172


Design Notes

The sequence was extracted from the pET21a-IleRS-His plasmid and expressed in the BL21 (DE3) E.coli strain. Codon optimization : 1179 from G to C Second stop codon was added in compliance with iGEM standards.


Source

Sebastian Maerkl Lab at EPFL, Switzerland. http://www.addgene.org/124112/

References