Coding
Cyt1Aa

Part:BBa_K2938003:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-17)
Revision as of 17:49, 17 September 2019 by Registry (Talk | contribs)

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Cyt1Aa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.Coli


Source

Isolated from pVE4-ADRC plasmid by PCR.

References