Coding
Cry4Ba

Part:BBa_K2938002:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-17)
Revision as of 17:42, 17 September 2019 by Registry (Talk | contribs)

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Cry4Ba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal NheI site found at 69
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal XbaI site found at 1681
    Illegal SpeI site found at 2370
    Illegal PstI site found at 185
    Illegal NgoMIV site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


Design Notes

None


Source

Isolated from Bacillus thuringiensis subsp. Israelensis by PCR.

References