Composite

Part:BBa_K2933185

Designed by: Jian Dong   Group: iGEM19_TJUSLS_China   (2019-09-16)
Revision as of 03:35, 17 September 2019 by Registry (Talk | contribs)

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T7 promoter+RBS b+linker h+His+Linker a+Sumo+Linker b+AFM-1+T7 terminator

The part consists of T7 promoter,RBS and protein coding(His+Linker a+Sumo+Linker b+AFM-1)and the biological module can be built into E.coil for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 390
    Illegal NheI site found at 167
    Illegal NheI site found at 1301
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 390
    Illegal BglII site found at 279
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None