Reporter
PLac_RFP

Part:BBa_K3128009

Designed by: Lucas PINERO   Group: iGEM19_Grenoble-Alpes   (2019-09-16)
Revision as of 07:44, 16 September 2019 by Registry (Talk | contribs)

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RFP protein under PLac promoter (with two restriction sites around the reporter)

The colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.

  • LacI sensitive
  • CAP sensitive

Two restrictions sites were added on both sides of the RFP gene : 5'BglII_RFP_BamHI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 229
    Illegal BamHI site found at 913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 789
    Illegal AgeI site found at 901
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
//classic/reporter/pret
//function/reporter/color
//function/reporter/pigment
Parameters
emissionRFP
excitation
tagNone