Reporter

Part:BBa_K3128004:Design

Designed by: Lucas PINERO   Group: iGEM19_Grenoble-Alpes   (2019-07-08)
Revision as of 17:03, 15 September 2019 by Pinerol (Talk | contribs) (→‎NanoLuciferase rapporteur under lactose promoter for BACTH assay)


NanoLuciferase reporter for BACTH assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the BBA J04450 for its CAP dependant Lactose Promoter (PLac) and it's strong RBS making it able to produce a large amount of protein when activated by IPTG and cAMP-CAP complexes. and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.

Source

PLac-RBS and the termintors are from iGEM plates from Bba_J04450 The NanoLuc gene is from Promega vector's

References