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2019 iGEM team Linkoping Sweden

2019 iGEM team Linkoping Sweden validated this part.

To test the bindning capacity of CBD-asPink (BBa_K3182000) we placed a white microbial cellulose bandage into the sonicated lysate of BL21(DE3) containing(?) the fusion protein see Figure 1</b> and let it incubate for 30 minutes. The bandage was then washed thrice in 70% ethanol which confirmed that the bindning of CDB-asPink was still intact after the washes to the bandage.

<b>Figure 1.Lysate (via sonication) from BL21 E. coli was incubated with Epiprotect (microbial cellulose bandage) for 1h and washed thrice with 70% ethanol.]

Test of the bindning ability of CBD-asPink (BBa_K3182000) to a bandage made of microbial cellulose in a PBS buffer solution (Figure 1).

Figure 2.

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