Coding
GluRS

Part:BBa_K2916002

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-24)
Revision as of 10:15, 24 July 2019 by Registry (Talk | contribs)

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GluRS protein equipped with a 6x HIS affinity tag

Asparaginyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.The His tag is used

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1297
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 328


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Categories
Parameters
None