Part:BBa_J100459:Experience

After adjusting the fluorescence over absorbance values obtained from the Synergy machine using scarlet fluorescent protein (SFP) wavelengths, it was observed that all three experimental samples showed significant difference compared to the negative control (GFP) and the promoter strength was almost twice as strong as the positive control (SFP).

The fluorescence over absorbance values were also adjusted using GFP wavelengths. GFP served as the positive control, with SFP as the negative control. All three experimental samples showed significant difference compared to the negative control, with experimental 1 showing no significant difference compared to the positive control. The error bars show standard error.

Data from the graphs show that the Staphylococcal enterotoxin B promoter express strong promoter strength for both gene expressions of SFP and GFP. DNA sequencing further confirmed that the promoter is a bidirectional promoter.

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