RNA

Part:BBa_K2624004:Experience

Designed by: Shuyao Zhou   Group: iGEM18_CPU_CHINA   (2018-08-26)
Revision as of 03:43, 8 December 2018 by KKehuandiCPU (Talk | contribs) (→‎Applications of BBa_K2624004)


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Applications of BBa_K2624004

We used this part to construct the "p-H*-U6" plasmid in order to express the inhibitory strand to inhibit the effect of RNA interference caused by miRNA. The results of qPCR (Real-time Quantitative Polymerase Chain Reaction) shows that the effect of inhibitory strand was obvious, which reduced the expression of miRNA and increase the expression of MAP4K4 mRNA.

20181017205344%21T--CPU_CHINA--hp-qidongzi.png

Figure. The expression of MAP4K4 miRNA (A) and MAP4K4 mRNA (B)

"p-H*" contains "HULC-pri-miRNA" parts; "p-H*-U6" contains "HULC-pri-miRNA-U6-pSil" parts;

"p-H" contains "HULC--pri-miRNA" parts; "Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--pri-miRNA-U6-pSil-TRE-sponge" parts;

User Reviews

UNIQbaf53cda9c5f1ca5-partinfo-00000000-QINU UNIQbaf53cda9c5f1ca5-partinfo-00000001-QINU