Composite
ADH2-kivD-

Part:BBa_K2588025:Design

Designed by: Dominika Wawrzyniak, Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)
Revision as of 03:56, 18 October 2018 by BKlopprogge (Talk | contribs) (→‎Design Notes)

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3-methyl-1-butanol synthesis module, arabinose-induced


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 7384
    Illegal XhoI site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 965
    Illegal AgeI site found at 6122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7210
    Illegal SapI.rc site found at 4042


Design Notes

non

Source

Composite of registry parts, genes amplified by PCR from E. coli genomic DNA, and synthetic DNA based on codon-optimized genes from Lactococcus lactis and Saccharomyces cerevisiae.

References