Coding

Part:BBa_K2653000:Design

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-07)
Revision as of 03:27, 18 October 2018 by Helen M (Talk | contribs) (→‎References)

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trim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 873
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1251


Design Notes

Trim21 has a special crab-shaped domain that can bind with the B30.2 domain of the Fc. Thus, with conservative Fc of the antibody binding with trim21, it then functions as a E3 ubiquitin ligase and proceeds the ubiquitin process of the antibody-antigen complex. Afterwards the proteasome depletes the target protein along with its antibody.

Source

The sequence of trim21 comes from the article: James L C, Keeble A H, Khan Z, et al. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(15):6200-6205.

References

1]Clift D, Mcewan W A, Labzin L I, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins[J]. Cell, 2017, 171(7):1692-1706.e18. [2]Mcewan W A, Falcon B, Vaysburd M, et al. Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(3):574.