Part:BBa_K2653000

trim21

Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.

Usage and Biology

The Tripartite motif-containing protein 21(Trim21) belongs to the E3 ubiquitin ligase family, which is encoded by the TRIM21 gene.The C-terminal B30.2 domain on trim21 shapes like a crab and offers a site for the conservative Fc region of human IgG 1,2 and 4 to bind with. This makes trim21 a potential protein degradation tool---when it binds with hIgG1-Fc, and after the antibody-antigen interaction, a ternary trim21-antibody-antigen complex would be built up. The trim21 then functions as a E3 ubiquitin ligase and proceeds the complex to be depleted through the ubiquitin-proteasome pathway.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 204
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 873
  Illegal BamHI site found at 1411
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 161
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1251
  

Source

The sequence of trim21 comes from the article: James L C, Keeble A H, Khan Z, et al. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(15):6200-6205.

通常

There are 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.

Source

Its sequence come from articles.