Coding

Part:BBa_K2653016:Design

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-12)
Revision as of 00:35, 18 October 2018 by Helen M (Talk | contribs) (→‎Design Notes)


GFP-nano-IgG1-FC-HA TAG-Trim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3482
    Illegal BamHI site found at 4020
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2770
    Illegal AgeI site found at 1615
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721
    Illegal BsaI site found at 2125
    Illegal BsaI site found at 3860
    Illegal SapI.rc site found at 2473


Design Notes

Choosing nanobody as the ideal antibody in our project aims to avoid the disadvantage of traditional antibodies, among which the most difficult is the large sequence amount of these antibodies that may influence the transfection or the expression of the part. Linking the nanobody of GFP and the hIgG1-Fc makes a promising recombinant antibody that is both recognizable by trim21 and small enough to be inserted into the plasmid.
P2A in our design aims to achieve the automatic cleavage of two peptides , offering a convenient way to express two proteins only with one promoter.
3XGS is the improvement we made this year, based on a 2XGS linker(). Besides the increased length, we made changes on the sequence of the middle GGGGS, so that the 3XGS could be more flexible and more specific in PCR and other aspects.

Source

dd

References