Coding

Part:BBa_K2534018:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM18_AFCM-Egypt   (2018-09-24)
Revision as of 00:21, 18 October 2018 by MTarek (Talk | contribs)

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kozak eGFP [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part by using eGFP for experimental characterization of lentiviral transfer plasmid transfection efficacy and apoptotic effect on colorectal cancer cell line RKO. This part is also an improved version of EGFP at BBa_I714891 by composting the kozak sequence into it for purposes of lentiviral delivery.

GFPAFCM18.png

Chkozakegfpafcm.png



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been synthesized through IDT offer


Source

http://2018.igem.org/Team:AFCM-Egypt/Bioinformatics_Page_link