Coding

Part:BBa_K2560082:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-20)
Revision as of 00:06, 18 October 2018 by Henselt (Talk | contribs) (→‎Source)


Phytobrick version of CDS Dummy


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward oligo: CTCGAATGGGTAAATCCCACTGATAGTAACCCGTTTTCTGACTGCGTAAGGCGACGACGCTT

Reverse Oligo: CTCAAAGCGTCGTCGCCTTACGCAGTCAGAAAACGGGTTACTATCAGTGGGATTTACCCATT

Source

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The DNA were designed with the <a href="https://pubs.acs.org/doi/full/10.1021/sb4001323?src=recsys">R2O DNA Designer</a>.

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