Part:BBa_K2717023:Experience

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Applications of BBa_K2717023

We used In-Fusion Cloning tech to link our composite to pUCyder. The whole pathway can be described in Figure 1.

Figure 1 the constuction of part BBa_K2717018

Then the plasmid is transfered into E.coli BL21. We use Flow Cytometer (FCM) to mearsure the bacteria's number and a relative fluorescence. Figure 2 is one of the FCM result graphs. The specific experiments and data processing methods can be found in our wiki. Finally, by comparing the final relative fluorescent intensity with that of control group, we found the exogenous CI sequence causes a 150% improvement to pink fluorescent intensity. That is to say, CI protein can indeed increase the expression of following gene.

Fig.18 A) The western blot image of CI, marker on the right. The protein is between 34kD and 25kD, which is precisely the exact molecular weight of itself. B) Data of the induction test. Each row indicates one time point and the columns from left to right represent IEtot of experiment strain, IEtot of the control strain and CI effect respectively. C) Bar gram drawn using the data presented in c to give a more intuitive impression.

User Reviews

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