Regulatory

Part:BBa_K2534015:Experience

Designed by: Mohammad Tarek Mansour   Group: iGEM18_AFCM-Egypt   (2018-09-24)
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Applications of BBa_K2534015

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines. 

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


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