Part:BBa_K2569032
PampC -mRFP
The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 631
Illegal AgeI site found at 743 - 1000COMPATIBLE WITH RFC[1000]
Improvement by replacing induced promoter with constitutive promoter
[http://2018.igem.org/Team:SKLMT-China Team SKLMT-China 2018] Improved the part(BBa_J04450)by replacing induced
promoter with constitutive promoter
lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG
and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP,
as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in <latin>Pseudomonas fluorescens </latin>pf-5.
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