Composite

Part:BBa_K2842690

Designed by: Catherine Fan, Eloise Nee   Group: iGEM18_UCL   (2018-10-02)
Revision as of 17:24, 17 October 2018 by Catfan (Talk | contribs)


Intein Monomer 2: GFP reporter flanked with orthogonal inteins

Intein Monomer 2
Function Standardised blue-white screening
Use in E. coli cells
Chassis Tested DH5α cells, BL21* cells
Abstraction Hierarchy Composite Device
Related Device BBa_K2842680
RFC standard RFC10,RFC12,RFC21,RFC23
& RFC25 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:UCL UCL iGEM 2018]


Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690
*edited to show relevant bands

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1536
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 1151
    Illegal SapI.rc site found at 422


[edit]
Categories
Parameters
None