Coding
S-OmpA

Part:BBa_K2632009

Designed by: Lingyu Zhong   Group: iGEM18_HZAU-China   (2018-09-16)
Revision as of 15:35, 17 October 2018 by StephanieHWX (Talk | contribs)

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OmpA coding sequence in Salmonella(Para-OmpA-RGD).

We use BBa_J36850 (Lpp-OmpA(46-159)-Streptavidin wild-type) to do the improvement. We add the RGD sequence at the same position of that in Salmonella, which can display the RGD on the outside of OmpA.

Reference

Park, S. H. et al. RGD peptide cell-surface display enhances the targeting and therapeutic efficacy of attenuated Salmonella-mediated cancer therapy. Theranostics 6, 1672–1682 (2016).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None