Composite

Part:BBa_K2686005:Experience

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-07)
Revision as of 12:50, 17 October 2018 by Caffeine4Life (Talk | contribs)

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The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.

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UNIQ60e2f26c6416574d-partinfo-00000001-QINU UNIQ60e2f26c6416574d-partinfo-00000002-QINU