Composite

Part:BBa_K2686006

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-08)
Revision as of 11:17, 17 October 2018 by Caffeine4Life (Talk | contribs)


Encapsulin protein with HexaHistidine insert, OT1 peptide at C terminus and a sfGFP-tag protein.

This Composite part consists of an encapsulin protein with a HexaHistidine linker and an OT1 peptide fused to the C terminus, followed by a T7 RBS and a sfGFP protein with a 15 amino acid long peptide tag. This peptide tag allows the sfGFP to bind to the encapsulin's interior surface, thus allowing the loading of sfGFP in the Encapsulin multimer. This would allow having a fluorescent Encapsulin protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 902
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 902
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457
    Illegal SapI.rc site found at 955


[edit]
Categories
Parameters
None