Composite

Part:BBa_K2686006:Experience

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-08)
Revision as of 11:14, 17 October 2018 by Caffeine4Life (Talk | contribs)

The part was designed and constructed in pET14 vector under a T7 promoter and terminator. The sequence was then confirmed using sanger sequencing. The part was expressed using a TX-TL BL21 DE3 cell free expression system. After expression and purification, the part was validated using a native gel, with multiple positive and negative controls.


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UNIQa4a4d65bfddf0893-partinfo-00000000-QINU UNIQa4a4d65bfddf0893-partinfo-00000001-QINU