Translational_Unit

Part:BBa_K2705005:Design

Designed by: Danqing Tong   Group: iGEM18_NKU_CHINA   (2018-09-30)
Revision as of 06:09, 17 October 2018 by Tong Danqing (Talk | contribs)


PgltAB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was applied to test the repression function of GltC to PgltAB, for which we applied different glutamate level and tested the fluorescence level changes.


Source

PgltAB is acquired from LL3 by PCR, GFP sequence is from our lab.

References