DNA
FRT

Part:BBa_J72001

Designed by: John Anderson   Group: Anderson Lab   (2008-07-15)
Revision as of 05:03, 16 July 2008 by JCAnderson (Talk | contribs)

{FRT} recombination site for flp recombinase in BBb DNA substate for the flp recombinase. This part has been successfully used to construct a variety of excisable genomic knockin/knockout cassettes. By transforming a cell with helper plasmid pCP20 (see Datsenko/Wanner paper), cassettes flanked by FRT sites are excised by recombination.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 18
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/flp
Parameters
None