Regulatory

Part:BBa_K2569011:Design

Designed by: wenxue Zhao   Group: iGEM18_SKLMT-China   (2018-09-27)
Revision as of 17:13, 16 October 2018 by DDY (Talk | contribs) (→‎Design Notes)

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PpltR, constitutive promoter in Pseudomonas. fluorescence pf-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter PpltR is a normal constitutive promoter that can be used in regulating the gene expression in Pseudomonadaceae.

Source

It is taken from the upstream region of the gene pltR ,whose length is 70bp.It is one of the constitutive promoters of pseudomonas fluorescence promoter library,its strength is characterized by a reporter gene ,firefly luciferase.It can be used to regulate the gene expression in Pseudomonadaceae through 3A Assembly.

References