Coding
bglx

Part:BBa_K2559002

Designed by: Yinpin Huang   Group: iGEM18_SCAU-China   (2018-10-07)
Revision as of 09:09, 16 October 2018 by Weiteng (Talk | contribs)


Bacterial cellulose synthase X(BglX)

The BBa_K2559001 is coding a β-glucosidase (BglX) which is involved in the bcs operons.


Usage and Biology

BGLX is a β-glucosidase which participates the cyanoamino acid metabolism (ko00460), starch and sucrose metabolism (ko00500), phenylpropanoid biosynthesis (ko00940), metabolic pathways (ko01100), biosynthesis of secondary metabolites (ko01110). The enzyme is expected to participate in hydrolysis of bacterial cellulose, instead of synthesis. Its role in cellulose biosynthesis remains to be further explored.

Figure 1 The pathways involve bcsH（entry 3.2.1.21） in starch and sucrose metabolism(ko00500)

We transfered bcsZ,H, A, B, C, D, bglX from the Acetobacter xylinum which are involved in the process of bacterial cellulose synthesis into the cyanobacteria.

Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement

Measurement of cyanobacteria glucose (3 repeats). The same of amount of transgenic cyanobacteria with bcsZH-ABCD-bglX genes and wild-type were treated with lysozyme to break the cells. Due to lacking a direct way to measure the content of cellu;ose in bacterial cell wall. Therefore, glucose can be used as an alternative parameter for measuring the content of cellulose since it can be digested into glucose by cellulose. The differences between the red c and blue column indicated that the content of bacteria cellulose.


Figure 2 The measurement of cellulose content in transgenic cyanobacteria with bcs gene.

The parts can further facilitate the in-depth research for other teams!

Reference :

1.Romling U & Galperin MY (Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. (Translated from eng) Trends Microbiol 23(9):545-557 (in eng).

2.Mazur O & Zimmer J (Apo- and cellopentaose-bound structures of the bacterial cellulose synthase subunit BcsZ. (Translated from eng) J Biol Chem 286(20):17601-17606 (in eng).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 516
    Illegal XhoI site found at 1206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 339


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