Composite

Part:BBa_K2718011

Designed by: Dimitri Popoff Sarah Sharkaoui Celia Guerin   Group: iGEM18_Aix-Marseille   (2018-08-27)
Revision as of 05:56, 16 October 2018 by Sowan (Talk | contribs)


IPTG inductible promotor RBS (strong) HmaS

Usage and Biology

HmaS is used in the metabolic pathway shown in figure 1 to produce benzyl alcohol. HmaS catalyzes the oxidative decarboxylation of phenylpyruvate to produce (S)Mandelate.


T--Aix-Marseille--BenzylAlcohol_pathway.png Figure 1 Metabolic pathway to produce benzyl alcohol


Production

We producted HmaS in E.coli DH5alpha, with 1mm IPTG at OD 0.8, 3 hours ' Image Blue Comassis

Figure 2 SDS-PAGE of HmaS production with and without induction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 607
    Illegal BamHI site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 556


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Parameters
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