Part:BBa_K2598027

bFMO(bacterial flavin-containing monooxygenase)

This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 157
  Illegal AgeI site found at 180
  Illegal AgeI site found at 484
  Illegal AgeI site found at 814
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Figure 1 shows results of gel electrophoresis of various parts after PCR with primer VF2\VR. The distance between primer binding sites and both ends of the parts are approximately 150 bp, thus rendering the product about 300 bp longer. The picture is edited to show a more compact photo.

File:T—UCAS-China—figure6 2018.tif

Characterization

Figure 2 shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.

Figure 2: Colors produced by enzymes that can combine with substrate in the medium