Coding
BcsH

Part:BBa_K2559001

Designed by: Yinpin Huang   Group: iGEM18_SCAU-China   (2018-10-07)
Revision as of 17:49, 15 October 2018 by Weiteng (Talk | contribs) (→‎Usage and Biology)

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Bacterial cellulose synthase H (BcsH)

The BBa_K2559002 is coding a cellulose complement protein A (CCPA) which is also regarded as Bacterial cellulose synthase H (BCSH).

Usage and Biology

BCSH is firstly identified in the Komagataeibacter andGluconacetobacter and is required for the activity of Bacterial cellulose synthase (BCS) in K. xylinus and Komagataeibacter hansenii.It has been shown to interact with BCSD, and participates in assisting the arrangement of glucan chains into crystalline ribbons in periplasm. The functions of BCSH accounts for the extremely high activity of BCS in K. xylinus.

Figure 1 The enzyme digestion of the biobrick with EcoRI and PstI

We introduced bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria.

Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement

Measurement of cyanobacteria glucose (3 repeats). The same of amount of transgenic cyanobacteria with bcsZH-ABCD-bglX genes and wild-type were treated with lysozyme to break the cells. Due to lacking a direct way to measure the content of cellu;ose in bacterial cell wall. Therefore, glucose can be used as an alternative parameter for measuring the content of cellulose since it can be digested into glucose by cellulose. The differences between the red column and blue column indicated that the content of bacteria cellulose.

Figure 2 The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group and control group.

The part is facilitate the in-depth research for other teams!

Reference :

1.Romling U & Galperin MY (Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. (Translated from eng) Trends Microbiol 23(9):545-557 (in eng).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 746
    Illegal AgeI site found at 907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 351


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