Part:BBa_K2680119

3G CI-repressible promoter

CI-repressible promoter BBa_R0051 flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

"The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein). [...] Intrinsic noise value: 0.0869 (compare with R0010: 0.0707; R0011: 0.0040). See [http://2015.igem.org/Team:William_and_Mary William_and_Mary iGEM 2015]."¹

Usage and Biology

"Strong promoter."¹

References

¹Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051

Elowitz repressilator paper: Elowitz, M. B., & Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 403(6767), 335-338. doi:10.1038/35002125

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 41
  Illegal BsaI.rc site found at 106