Composite

Part:BBa_K2217024

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-25)
Revision as of 21:48, 14 October 2018 by MTarek (Talk | contribs)


CMV Enhancer + CMV Promoter

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part through gel electrophoresis for experimental characterization of lentiviral transfer plasmid transfection efficacy as a strong promoter enhanced by fusing CMV enhancer. https://parts.igem.org/File:AFCMCMV.png

Egypt-AFCM Team Improvement

[http://2017.igem.org/Team:AFCM-Egypt# Egypt-AFCM Team] improved this part BBa_K1119006 by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activity BBa_K2217024. Part characterization and usage can be found at Part Experience


Usage and Biology

Part BBa_K2217022 was composed of CMV Enhancer as well as CMV Promoter (BBa_K2217000 and BBa_K2217006 respectively) as constitutive promoters to enhance the transcription process of the laci that was submitted BBa_K2217012

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection
Parameters
None