Part:BBa_K2680123

3G pTlpA

pTlpA promoter BBa_K2500003 in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacI^Q) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."¹ See pTlpAr BBa_K2680124 for the reverse complement of pTlpA. pTlpA is flanked by prefix sticky end A and suffix sticky end B.

References

¹Piraner, D. I., Abedi, M.H., Moser, B.A., Lee-Gosselin, A., & Shapiro M. G. Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology doi:10.1038/nchembio.2233, 2 (2016).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 41
  Illegal BsaI.rc site found at 109