RBS

Part:BBa_K2541101:Design

Designed by: Shuting Zheng   Group: iGEM18_Jilin_China   (2018-10-05)
Revision as of 06:33, 13 October 2018 by Shuting (Talk | contribs) (→‎Design Notes)

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Heat-repressible RNA-based thermosensor-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 31


Design Notes

The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.

Source

This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.

References

[1]Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.

[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.