Regulatory

Part:BBa_K091100:Design

Designed by: Pallavi Penumetcha   Group: iGEM08_Davidson-Missouri_Western   (2008-05-29)
Revision as of 14:09, 2 June 2008 by Pallavi (Talk | contribs) (→‎Design Notes)

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pLac_lux hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This truth table illustrates PoPS output if IPTG, LacIq, HSL and LuxR inputs are all varied.
PLac Lux logic.jpg
This truth table illustrates PoPS output if the lacIq and luxR genes are constitutively active and AHL and IPTG input is varied.
PLac lux promoter.jpg
Other Design Notes:
Deleted the CAP binding site in R0010. Also designed by Robert Cool and Erin Feeney.

Source

R0010 modified with the insertion of a lux box in between -35 and -10.

References