Regulatory

Part:BBa_K2680524

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-12)
Revision as of 01:22, 13 October 2018 by Jaurban (Talk | contribs)


pLac/ara-1

pLac/ara-1 dual input promoter. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 425
    Illegal XhoI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None