Composite
Part:BBa_K2656100
Designed by: Adrián Requena Gutiérrez, Carolina Ropero Group: iGEM18_Valencia_UPV (2018-09-22)
Revision as of 17:58, 12 October 2018 by Carolinarp (Talk | contribs)
sfGFP transcriptional unit 1
Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
- BBa_K2656008: the J61100 very weak ribosome biding site in its Golden Braid compatible version
- BBa_K2656013: the BBa_I746916 sfGFP sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77
[edit]
Categories
Parameters
| None |