Part:BBa_K2686006:Experience

The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a BL21 DE3 cell free expression system. After expression and purification, the part was validated using a native gel, with multiple positive and negative controls (review iGEM EPFL 2018 results page).

The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.

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