Part:BBa_K2656100

sfGFP transcriptional unit 1

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 promoter in its Golden Braid compatible version
• BBa_K2656008: the B0030 ribosome biding site in its Golden Braid compatible version

• </html> BBa_K2656013: BBa_I746916 sfGFP sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 77