Part:BBa_K2686005

Encapsulin protein with HexaHistidine insert & sfGFP under native promoter included between BsaI cut

This Encapsulin protein was first modified by insertion of a hexahistidine insert to increase heat stability, and endow better hydrodynamic properties (review part BBa_K2686000). After the encapsulin site on the plasmid (After the C-terminus), is a site for sfGFP under native promoter included within BSAI cut sites. These BSAI cut sites would allow for the rapid, scarless introduction of antigen peptides which will be expressed on the surface of the Encapsulin subunit (After the C-terminus). The insert in between the two BsaI cut sites, consisting of sfGFP with a native promoter and terminator, allows for checking the success of the insertion of the antigen (green colonies do not contain the desired peptide insert).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 77
  Illegal BglII site found at 492
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 426
  Illegal SapI.rc site found at 457
  Illegal SapI.rc site found at 1031