Coding
LaG2

Part:BBa_K2549002:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 13:12, 12 October 2018 by Wave (Talk | contribs)

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anti-GFP (LaG2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. LaG2 fuses two copies of LaG16 (Part:BBa_K2549002) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP[1].

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170