Coding

Part:BBa_K2617006

Designed by: Changyu Li   Group: iGEM18_UESTC-China   (2018-09-30)
Revision as of 12:35, 10 October 2018 by Yet (Talk | contribs)


GroEL

Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 880
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.

Fig. 1 The measurement of optical density (A600) by periodically taking samples.


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