Coding

Part:BBa_K2617000

Designed by: Yetao Zou   Group: iGEM18_UESTC-China   (2018-09-30)
Revision as of 10:34, 10 October 2018 by Yet (Talk | contribs) (→‎Enzyme activity)


Xyn10D-Fae1A：Bifunctional xylanase/ferulic acid esterase

Xyn1D-fae1A cuts off the crosslinking between polysaccharides and lignin in cell wall and degrade xylose hemicellulose. This part is responsible for pretreatment of straw.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 381
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1236

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 726 amino acids with a molecular mass of 82.3 kDa.

Enzyme activity

Fig. 1 Standard Curve of Xylose. Data were measured in buffer at pH 6.0 and 40℃.
Fig. 2 Xylanase Activity on Xylan. Data were measured in buffer at pH 6.0 and 40℃.


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyPrevotella ruminicola