Composite

Part:BBa_K2842710

Designed by: Catherine Fan, Eloise Nee   Group: iGEM18_UCL   (2018-10-08)
Revision as of 16:42, 9 October 2018 by Catfan (Talk | contribs)


NikR with TerL-C intein

E. coli nickel binding protein, NikR, assembled into BBa_K2842669 (Intein Passenger).

Figure 1: colony PCR of functionalised Intein Passenger BBa_K2842669

(1) NikR with TerL-C intein BBa_K2842710
(2) ER protein with TerL-C intein BBa_K2842720
*edited to show relevant bands

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 771
    Illegal BsaI.rc site found at 28


[edit]
Categories
Parameters
None