E. coli transformation

Revision as of 19:54, 20 March 2008 by Msicchio (Talk | contribs)

Using the puch tool, puch out the appropriate DNA. Between puches

Add 2µL of DNA in TE to 50µL of competent cells (TOP10)

Allow the DNA and competent cells to sit on ice for 20 minutes

Heat shock at 42ºC for 60 sec in water bath.

Recover on ice for 5 min.

Add 200 µL SOC media

Incubate at 37ºC for 2 hr while the tubes are rotating.